By introducing the target gene into the bacteria to express it to get desired proteins, we get the transgenic bacterias. Since the proteins produced by fermentation are often ultimately used in the food industry, agriculture and healthcare. The safety of genetic bacterias is very important. The selection and designing of bacterias requires the following characteristics. Firstly, the bacterial fermentation process needs to have a high conversion rate and a high yield. Secondly, bacterias can use commonly used carbon sources for uninterrupted continuous fermentation. Thirdly, the strain is not a pathogenic bacteria, nor does it produce endotoxins. Fourthly, the metabolic process of the strain is artificially controllable. Fifthly, the genome of the strain can remain stable after proper DNA recombination.
In agriculture, genetically modified bacterias are mainly used in biological pesticides, crop fertilizers and pesticide degradation. For example, biological insecticides can be produced by introducing insecticidal genes. Such insecticides can kill pest larvae, but they are very safe for humans. Genetic engineering can also be used to increase the expression of nitrogen-fixing-related genes in rhizobia, so that crops can better absorb fertilizer. In addition, the use of chemical pesticides in the process of agricultural production can easily cause serious excess pesticide residues in agricultural products. Through genetic engineering screening of pesticide degradation genes, the efficient degradation of a variety of pesticides can be achieved.
Genetically engineered bacterias can be used as an overexpression platform for foreign proteins. Used in the production of medical vaccines, protein drugs, antibody drugs, and medical antibiotics.
Edible fermentation fungi in food production can also be modified through genetic engineering technology. In this way, the fermentation time is shortened, the fermented products are of high quality, and the yield of fermented foods is increased.
For example, by cultivating genetically modified bacterias that can degrade plastics and purify sewage. These environmentally friendly genetically engineered bacterias play an important role in the convenience of environmental protection.
Similar to transgenic animals and plants, the construction of transgenic bacterias is mainly divided into the following steps, including target gene acquisition, gene expression vector construction, target gene introduction into recipient cells, recipient cell proliferation, and transgene identification. Bacteria is a single-celled organism. On the one hand, you can use it as a host to assemble various types of target genes into a cell. On the other hand, you can use genetically modified bacterias to produce protein products. The high-throughput htDNA-chip® technology platform can be mainly applied to the screening and amplification of target genes and the results identification.
In terms of target gene screening, our silicon-based high-throughput htDNA-chip® platform can meet your target gene screening needs by constructing a DNA library with high efficiency. In terms of target gene amplification, the silicon-based high-throughput htDNA-chip® platform can achieve target gene amplification through high-throughput synthesis of nucleotide chains.
In order to confirm the successful integration of the target gene into the recipient cell, the final step of screening is required. Through the high-throughput next-generation sequencing technology of htDNA-chip®, it is possible to efficiently complete many sets of gene sequencing in a single run, which improves the efficiency of screening. Then you can select the successful transgenic bacterias for large-scale cultivation.
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